Publications
2026
Liu Y, Arús BA, Mishra K, Wang T, Zhang K, Luciano MP, Bandi VG, Kumar A, Guo Z, Guan Y, Bick MJ, Xu M, Lingg J, Bae J, Kang A, Gerben SR, Bera AK, Vaughan JC, Manton JD, Derivery E, Schnermann MJ, Stiel AC, Bruns OT, Xu C, Baker D. De Novo Design of Near-Infrared Fluorescence-Activating Proteins. J Am Chem Soc.. 2026 10.1021/jacs.5c195942025
Ludwig Englert, Bjarne Perleberg, Shilpa Tayal, Bingzhi Wang, Yishu Huang, Azeem Mohammed, Hailong He, Andre C. Stiel, Vasilis Ntziachristos. Assessment of performance characteristics of photoswitchable bacteriophytochromes at different depths using optoacoustic mesoscopy. Optics Express. 2025 10.1364/oe.564945Bianca Menchicchi, Andre C. Stiel, Mattia Nieddu, J. P. Fuenzalida-Werner. Fluorescent proteins: A journey from the cell to extreme environments in material science. Photochemistry and Photobiology. 2025 10.1111/php.70011
Juan Aguirre, Christine Gasteiger, Benedikt Hindelang, Markus Seeger, Andrei Bereznhoi, Ina Weidenfeld, Ulf Darsow, Andre C. Stiel, Susanne Annette Steimle-Grauer, Christian Posch, Tilo Biedermann, Vasilis Ntziachristos. Non-invasive characterization of melanoma depth at single-cell resolution. Journal of the European Academy of Dermatology and Venereology. 2025 10.1111/jdv.20811
Gollner S, Colditz M, Huang Y, Schmidt H, Winkler A, Stiel AC. Chip-Based Optoacoustic Single-Cell Detection in Flow Using Point-Source Optimized Surface Acoustic Wave Transducers. ACS Applied Materials & Interfaces. 2025 10.1021/acsami.4c20182
Liu Y, Chuah J, Huang Y, Stiel AC, Unser M, Dong J. Model-based temporal unmixing towards quantitative photo-switching optoacoustic tomography. Optics Express. 2025 10.1364/OE.550253
Huang Y, Stankevych M, Gujrati V, Klemm U, Mohammed A, Wiesner D, Saccomano M, Tost M, Feuchtinger A, Mishra K, Bruns O, Geerlof A, Ntziachristos V, Stiel AC.. Photoswitching protein-XTEN fusions as injectable optoacoustic probes. Acta Biomater. 2025 10.1016/j.actbio.2025.02.002
2024
Xu C, Liu Y, Arús B, Mishra K, Luciano M, Bandi V, Kumar A, Guo Z, Bick M, Xu M, Zhang K, Lingg J, Bae J, Kang A, Bera A, Vaughan J, Manton J, Derivery E, Schnermann M, Stiel AC, Bruns O, Guan Y, Baker D. De novo Design of Near Infrared Fluorescent Proteins. Research Square. 2024 10.21203/rs.3.rs-4652998/v1Stiel AC, Ntziachristos V.. Controlling the sound of light: photoswitching optoacoustic imaging. Nat Methods. 2024 10.1038/s41592-024-02396-2
Kröger P, Stiel AC, Stüven B, Shanmugaratnam S, Schoch S, Wachten D, Höcker B.. A fluorescent biosensor for the visualization of agmatine. bioRxiv. 2024 10.1101/2024.07.27.605435
Huang S, He H, Tom RZ, Glasl S, Anzenhofer P, Stiel AC, Hofmann SM, Ntziachristos V.. Non-invasive optoacoustic imaging of dermal microcirculatory revascularization in diet-induced obese mice undergoing exercise intervention. Photoacoustics. 2024 10.1016/j.pacs.2024.100628
Arús BA, Yiu J, Lingg JGP, Hofmann A, Fumo AR, Ji H, Jethwa C, Park RK, Henderson J, Mishra K, Mukha I, Stiel AC, Santovito D, Weber C, Reeps C, Rohm M, Bartelt A, Valdez TA, Chmyrov A, Bruns OT. Macroscopic label-free biomedical imaging with shortwave infrared Raman scattering. bioRxiv. 2024 10.1101/2024.06.10.597863
2023
Liu N, Gujrati V, Werner JP, Mishra K, Anzenhofer P, Stiel AC, Mettenleiter G, Feuchtinger A, Walch A, Ntziachristos V. Bacterial outer membrane vesicles as cationic dye carriers for optoacoustics-guided phototherapy of cancer. Cancer Nano. 2023 10.1186/s12645-023-00191-wHasler M, Patrian M, Banda-Vázquez JA, Ferrara S, Stiel AC, Fuenzalida-Werner JP, Costa RD.. Fully Biogenic Near-Infrared Phosphors: Phycobiliproteins and Cellulose at Force Toward Highly Efficient and Stable Bio-Hybrid Light-Emitting Diodes. Adv. Funct. Materials. 2023 10.1002/adfm.202301820
Rodrigues EC, Stiel AC.. It’s a two-way street: Photoswitching and reversible changes of the protein matrix in photoswitchable fluorescent proteins and bacteriophytochromes. FEBS Lett. 2023 10.1002/1873-3468.14609
2022
Liu N, Mishra K, Stiel AC, Gujrati V, Ntziachristos V.. The sound of drug delivery: Optoacoustic imaging in pharmacology. Adv Drug Deliv Rev. 2022 10.1016/j.addr.2022.114506Mishra K, Fuenzalida-Werner JP, Pennacchietti F, Janowski R, Chmyrov A, Huang Y, Zakian C, Klemm U, Testa I, Niessing D, Ntziachristos V, Stiel AC.. Genetically encoded photo-switchable molecular sensors for optoacoustic and super-resolution imaging. Nat Biotechnol. 2022 10.1038/s41587-021-01100-5
2021
Fuenzalida-Werner JP, Mishra K, Stankevych M, Klemm U, Ntziachristos V, Stiel AC.. Alginate beads as a highly versatile test-sample for optoacoustic imaging. Photoacoustics. 2021 10.1016/j.pacs.2021.100301Yun M, You SH, Nguyen VH, Prakash J, Glasl S, Gujrati V, Choy HE, Stiel AC, Min JJ, Ntziachristos V.. Reporter gene-based optoacoustic imaging of E. coli targeted colon cancer in vivo. Sci Rep. 2021 10.1038/s41598-021-04047-4
Huang S, Blutke A, Feuchtinger A, Klemm U, Zachariah Tom R, Hofmann SM, Stiel AC, Ntziachristos V.. Functional multispectral optoacoustic tomography imaging of hepatic steatosis development in mice. EMBO Mol Med. 2021 10.15252/emmm.202013490
Liu N, Gujrati V, Malekzadeh-Najafabadi J, Werner JPF, Klemm U, Tang L, Chen Z, Prakash J, Huang Y, Stiel A, Mettenleiter G, Aichler M, Blutke A, Walch A, Kleigrewe K, Razansky D, Sattler M, Ntziachristos V.. Croconaine-based nanoparticles enable efficient optoacoustic imaging of murine brain tumors. Photoacoustics. 2021 10.1016/j.pacs.2021.100263
Herud-Sikimić O, Stiel AC, Kolb M, Shanmugaratnam S, Berendzen KW, Feldhaus C, Höcker B, Jürgens G.. A biosensor for the direct visualization of auxin. Nature. 2021 10.1038/s41586-021-03425-2
Seeger M, Stiel AC, Ntziachristos V.. In vitro optoacoustic flow cytometry with light scattering referencing. Sci Rep. 2021 10.1038/s41598-021-81584-y
Stankevych M, Mishra K, Ntziachristos V, Stiel AC.. A practical guide to photoswitching optoacoustics tomography. Methods Enzymol. 2021 10.1016/bs.mie.2021.06.031
2020
Deán-Ben XL, Weidenfeld I, Degtyaruk O, Ntziachristos V, Stiel AC, Razansky D.. Deep tissue volumetric optoacoustic tracking of individual circulating tumor cells in an intracardially perfused mouse model. Neoplasia. 2020 10.1016/j.neo.2020.06.008Fuenzalida Werner JP, Huang Y, Mishra K, Janowski R, Vetschera P, Heichler C, Chmyrov A, Neufert C, Niessing D, Ntziachristos V, Stiel AC.. Challenging a Preconception: Optoacoustic Spectrum Differs from the Optical Absorption Spectrum of Proteins and Dyes for Molecular Imaging. Anal Chem. 2020 10.1021/acs.analchem.0c01902
Mishra K, Stankevych M, Fuenzalida-Werner JP, Grassmann S, Gujrati V, Huang Y, Klemm U, Buchholz VR, Ntziachristos V, Stiel AC.. Multiplexed whole-animal imaging with reversibly switchable optoacoustic proteins. Sci Adv. 2020 10.1126/sciadv.aaz6293
Stiel AC, Shanmugaratnam S, Herud-Sikimic O, Jürgens G, Höcker B.. Ligand promiscuity in the tryptophan repressor – from structural understanding towards rational design. bioRxiv. 2020 10.1101/2020.01.20.912972
2019
Weidenfeld I, Zakian C, Duewell P, Chmyrov A, Klemm U, Aguirre J, Ntziachristos V, Stiel AC.. Homogentisic acid-derived pigment as a biocompatible label for optoacoustic imaging of macrophages. Nat Commun. 2019 10.1038/s41467-019-13041-4Fuenzalida Werner JP, Mishra K, Huang Y, Vetschera P, Glasl S, Chmyrov A, Richter K, Ntziachristos V, Stiel AC.. Structure-Based Mutagenesis of Phycobiliprotein smURFP for Optoacoustic Imaging. ACS Chem Biol. 2019 10.1021/acschembio.9b00299
Mokrousov MD, Novoselova MV, Nolan J, Harrington W, Rudakovskaya P, Bratashov DN, Galanzha EI, Fuenzalida-Werner JP, Yakimov BP, Nazarikov G, Drachev VP, Shirshin EA, Ntziachristos V, Stiel AC, Zharov VP, Gorin DA.. Amplification of photoacoustic effect in bimodal polymer particles by self-quenching of indocyanine green. Biomed Opt Express. 2019 10.1364/BOE.10.004775
Mishra K, Fuenzalida-Werner JP, Ntziachristos V, Stiel AC.. Photocontrollable Proteins for Optoacoustic Imaging. Anal Chem. 2019 10.1021/acs.analchem.9b01048
Peters L, Weidenfeld I, Klemm U, Loeschcke A, Weihmann R, Jaeger KE, Drepper T, Ntziachristos V, Stiel AC.. Phototrophic purple bacteria as optoacoustic in vivo reporters of macrophage activity. Nat Commun. 2019 10.1038/s41467-019-09081-5
Gujrati V, Prakash J, Malekzadeh-Najafabadi J, Stiel A, Klemm U, Mettenleiter G, Aichler M, Walch A, Ntziachristos V.. Bioengineered bacterial vesicles as biological nano-heaters for optoacoustic imaging. Nat Commun. 2019 10.1038/s41467-019-09034-y
2018
Fuenzalida-Werner JP, Janowski R, Mishra K, Weidenfeld I, Niessing D, Ntziachristos V, Stiel AC.. Crystal structure of a biliverdin-bound phycobiliprotein: Interdependence of oligomerization and chromophorylation. J Struct Biol. 2018 10.1016/j.jsb.2018.09.013Vetschera P, Mishra K, Fuenzalida-Werner JP, Chmyrov A, Ntziachristos V, Stiel AC.. Characterization of Reversibly Switchable Fluorescent Proteins in Optoacoustic Imaging. Anal Chem. 2018 10.1021/acs.analchem.8b02599
Before 2017, older publications of AC Stiel
2016
Stiel AC, Nellen M, Höcker B.. PocketOptimizer and the Design of Ligand Binding Sites. Methods Mol Biol. 2016 10.1007/978-1-4939-3569-7_52015
Deán-Ben XL, Stiel AC, Jiang Y, Ntziachristos V, Westmeyer GG, Razansky D.. Light fluence normalization in turbid tissues via temporally unmixed multispectral optoacoustic tomography. Opt Lett. 2015 10.1364/OL.40.004691Stiel AC, Deán-Ben XL, Jiang Y, Ntziachristos V, Razansky D, Westmeyer GG.. High-contrast imaging of reversibly switchable fluorescent proteins via temporally unmixed multispectral optoacoustic tomography. Opt Lett. 2015 10.1364/OL.40.000367
2014
Lavoie-Cardinal F, Jensen NA, Westphal V, Stiel AC, Chmyrov A, Bierwagen J, Testa I, Jakobs S, Hell SW.. Two-color RESOLFT nanoscopy with green and red fluorescent photochromic proteins. Chemphyschem. 2014 10.1002/cphc.201301016Stiel AC, Feldmeier K, Höcker B.. Identification of protein scaffolds for enzyme design using scaffold selection. Methods Mol Biol. 2014 10.1007/978-1-4939-1486-9_9
2011
Willig KI, Stiel AC, Brakemann T, Jakobs S, Hell SW.. Dual-label STED nanoscopy of living cells using photochromism. Nano Lett. 2011 10.1021/nl202290wBrakemann T, Stiel AC, Weber G, Andresen M, Testa I, Grotjohann T, Leutenegger M, Plessmann U, Urlaub H, Eggeling C, Wahl MC, Hell SW, Jakobs S.. A reversibly photoswitchable GFP-like protein with fluorescence excitation decoupled from switching. Nat Biotechnol. 2011 10.1038/nbt.1952
2010
Brakemann T, Weber G, Andresen M, Groenhof G, Stiel AC, Trowitzsch S, Eggeling C, Grubmüller H, Hell SW, Wahl MC, Jakobs S.. Molecular basis of the light-driven switching of the photochromic fluorescent protein Padron. J Biol Chem. 2010 10.1074/jbc.M109.0863142008
Testa I, Schönle A, von Middendorff C, Geisler C, Medda R, Wurm CA, Stiel AC, Jakobs S, Bossi M, Eggeling C, Hell SW, Egner A.. Nanoscale separation of molecular species based on their rotational mobility. Opt Express. 2008 10.1364/oe.16.021093Stiel AC, Andresen M, Bock H, Hilbert M, Schilde J, Schönle A, Eggeling C, Egner A, Hell SW, Jakobs S.. Generation of monomeric reversibly switchable red fluorescent proteins for far-field fluorescence nanoscopy. Biophys J. 2008 10.1529/biophysj.108.130146
Andresen M, Stiel AC, Fölling J, Wenzel D, Schönle A, Egner A, Eggeling C, Hell SW, Jakobs S.. Photoswitchable fluorescent proteins enable monochromatic multilabel imaging and dual color fluorescence nanoscopy. Nat Biotechnol. 2008 10.1038/nbt.1493
2007
Eggeling, C., Hilbert, M., Bock, H., Ringemann, C., Hofmann, M., Stiel, A. C., Andresen M, Jakobs S, Egner A, Schönle A, Hell, S. W. . Reversible photoswitching enables single‐molecule fluorescence fluctuation spectroscopy at high molecular concentration. Microscopy research and technique. 2007 10.1002/jemt.20505Egner A, Geisler C, von Middendorff C, Bock H, Wenzel D, Medda R, Andresen M, Stiel AC, Jakobs S, Eggeling C, Schönle A, Hell SW.. Fluorescence nanoscopy in whole cells by asynchronous localization of photoswitching emitters. Biophys J. 2007 10.1529/biophysj.107.112201
Andresen M, Stiel AC, Trowitzsch S, Weber G, Eggeling C, Wahl MC, Hell SW, Jakobs S.. Structural basis for reversible photoswitching in Dronpa. Proc Natl Acad Sci U S A. 2007 10.1073/pnas.0700629104
Stiel AC, Trowitzsch S, Weber G, Andresen M, Eggeling C, Hell SW, Jakobs S, Wahl MC.. 1.8 A bright-state structure of the reversibly switchable fluorescent protein Dronpa guides the generation of fast switching variants. Biochem J. 2007 10.1042/BJ20061401