2025

Gollner S, Colditz M, Huang Y, Schmidt H, Winkler A, Stiel AC. Simon Gollner, Melanie Colditz, Yishu Huang, Hagen Schmidt, Andreas Winkler, Andre C. Stiel. ACS Applied Materials & Interfaces. 2025
10.1021/acsami.4c20182

Liu Y, Chuah J, Huang Y, Stiel AC, Unser M, Dong J. Model-based temporal unmixing towards quantitative photo-switching optoacoustic tomography. Optics Express. 2025
10.1364/OE.550253

Huang Y, Stankevych M, Gujrati V, Klemm U, Mohammed A, Wiesner D, Saccomano M, Tost M, Feuchtinger A, Mishra K, Bruns O, Geerlof A, Ntziachristos V, Stiel AC.. Photoswitching protein-XTEN fusions as injectable optoacoustic probes. Acta Biomater. 2025
10.1016/j.actbio.2025.02.002

2024

Xu C, Liu Y, Arús B, Mishra K, Luciano M, Bandi V, Kumar A, Guo Z, Bick M, Xu M, Zhang K, Lingg J, Bae J, Kang A, Bera A, Vaughan J, Manton J, Derivery E, Schnermann M, Stiel AC, Bruns O, Guan Y, Baker D. De novo Design of Near Infrared Fluorescent Proteins. Research Square. 2024
10.21203/rs.3.rs-4652998/v1

Stiel AC, Ntziachristos V.. Controlling the sound of light: photoswitching optoacoustic imaging. Nat Methods. 2024
10.1038/s41592-024-02396-2

Kröger P, Stiel AC, Stüven B, Shanmugaratnam S, Schoch S, Wachten D, Höcker B.. A fluorescent biosensor for the visualization of agmatine. bioRxiv. 2024
10.1101/2024.07.27.605435

Huang S, He H, Tom RZ, Glasl S, Anzenhofer P, Stiel AC, Hofmann SM, Ntziachristos V.. Non-invasive optoacoustic imaging of dermal microcirculatory revascularization in diet-induced obese mice undergoing exercise intervention. Photoacoustics. 2024
10.1016/j.pacs.2024.100628

Arús BA, Yiu J, Lingg JGP, Hofmann A, Fumo AR, Ji H, Jethwa C, Park RK, Henderson J, Mishra K, Mukha I, Stiel AC, Santovito D, Weber C, Reeps C, Rohm M, Bartelt A, Valdez TA, Chmyrov A, Bruns OT. Macroscopic label-free biomedical imaging with shortwave infrared Raman scattering. bioRxiv. 2024
10.1101/2024.06.10.597863

2023

Liu N, Gujrati V, Werner JP, Mishra K, Anzenhofer P, Stiel AC, Mettenleiter G, Feuchtinger A, Walch A, Ntziachristos V. Bacterial outer membrane vesicles as cationic dye carriers for optoacoustics-guided phototherapy of cancer. Cancer Nano. 2023
10.1186/s12645-023-00191-w

Hasler M, Patrian M, Banda-Vázquez JA, Ferrara S, Stiel AC, Fuenzalida-Werner JP, Costa RD.. Fully Biogenic Near-Infrared Phosphors: Phycobiliproteins and Cellulose at Force Toward Highly Efficient and Stable Bio-Hybrid Light-Emitting Diodes. Adv. Funct. Materials. 2023
10.1002/adfm.202301820

Rodrigues EC, Stiel AC.. It’s a two-way street: Photoswitching and reversible changes of the protein matrix in photoswitchable fluorescent proteins and bacteriophytochromes. FEBS Lett. 2023
10.1002/1873-3468.14609

2022

Liu N, Mishra K, Stiel AC, Gujrati V, Ntziachristos V.. The sound of drug delivery: Optoacoustic imaging in pharmacology. Adv Drug Deliv Rev. 2022
10.1016/j.addr.2022.114506

Mishra K, Fuenzalida-Werner JP, Pennacchietti F, Janowski R, Chmyrov A, Huang Y, Zakian C, Klemm U, Testa I, Niessing D, Ntziachristos V, Stiel AC.. Genetically encoded photo-switchable molecular sensors for optoacoustic and super-resolution imaging. Nat Biotechnol. 2022
10.1038/s41587-021-01100-5

2021

Fuenzalida-Werner JP, Mishra K, Stankevych M, Klemm U, Ntziachristos V, Stiel AC.. Alginate beads as a highly versatile test-sample for optoacoustic imaging. Photoacoustics. 2021
10.1016/j.pacs.2021.100301

Yun M, You SH, Nguyen VH, Prakash J, Glasl S, Gujrati V, Choy HE, Stiel AC, Min JJ, Ntziachristos V.. Reporter gene-based optoacoustic imaging of E. coli targeted colon cancer in vivo. Sci Rep. 2021
10.1038/s41598-021-04047-4

Huang S, Blutke A, Feuchtinger A, Klemm U, Zachariah Tom R, Hofmann SM, Stiel AC, Ntziachristos V.. Functional multispectral optoacoustic tomography imaging of hepatic steatosis development in mice. EMBO Mol Med. 2021
10.15252/emmm.202013490

Liu N, Gujrati V, Malekzadeh-Najafabadi J, Werner JPF, Klemm U, Tang L, Chen Z, Prakash J, Huang Y, Stiel A, Mettenleiter G, Aichler M, Blutke A, Walch A, Kleigrewe K, Razansky D, Sattler M, Ntziachristos V.. Croconaine-based nanoparticles enable efficient optoacoustic imaging of murine brain tumors. Photoacoustics. 2021
10.1016/j.pacs.2021.100263

Herud-Sikimić O, Stiel AC, Kolb M, Shanmugaratnam S, Berendzen KW, Feldhaus C, Höcker B, Jürgens G.. A biosensor for the direct visualization of auxin. Nature. 2021
10.1038/s41586-021-03425-2

Seeger M, Stiel AC, Ntziachristos V.. In vitro optoacoustic flow cytometry with light scattering referencing. Sci Rep. 2021
10.1038/s41598-021-81584-y

Stankevych M, Mishra K, Ntziachristos V, Stiel AC.. A practical guide to photoswitching optoacoustics tomography. Methods Enzymol. 2021
10.1016/bs.mie.2021.06.031

2020

Deán-Ben XL, Weidenfeld I, Degtyaruk O, Ntziachristos V, Stiel AC, Razansky D.. Deep tissue volumetric optoacoustic tracking of individual circulating tumor cells in an intracardially perfused mouse model. Neoplasia. 2020
10.1016/j.neo.2020.06.008

Fuenzalida Werner JP, Huang Y, Mishra K, Janowski R, Vetschera P, Heichler C, Chmyrov A, Neufert C, Niessing D, Ntziachristos V, Stiel AC.. Challenging a Preconception: Optoacoustic Spectrum Differs from the Optical Absorption Spectrum of Proteins and Dyes for Molecular Imaging. Anal Chem. 2020
10.1021/acs.analchem.0c01902

Mishra K, Stankevych M, Fuenzalida-Werner JP, Grassmann S, Gujrati V, Huang Y, Klemm U, Buchholz VR, Ntziachristos V, Stiel AC.. Multiplexed whole-animal imaging with reversibly switchable optoacoustic proteins. Sci Adv. 2020
10.1126/sciadv.aaz6293

Stiel AC, Shanmugaratnam S, Herud-Sikimic O, Jürgens G, Höcker B.. Ligand promiscuity in the tryptophan repressor – from structural understanding towards rational design. bioRxiv. 2020
10.1101/2020.01.20.912972

2019

Weidenfeld I, Zakian C, Duewell P, Chmyrov A, Klemm U, Aguirre J, Ntziachristos V, Stiel AC.. Homogentisic acid-derived pigment as a biocompatible label for optoacoustic imaging of macrophages. Nat Commun. 2019
10.1038/s41467-019-13041-4

Fuenzalida Werner JP, Mishra K, Huang Y, Vetschera P, Glasl S, Chmyrov A, Richter K, Ntziachristos V, Stiel AC.. Structure-Based Mutagenesis of Phycobiliprotein smURFP for Optoacoustic Imaging. ACS Chem Biol. 2019
10.1021/acschembio.9b00299

Mokrousov MD, Novoselova MV, Nolan J, Harrington W, Rudakovskaya P, Bratashov DN, Galanzha EI, Fuenzalida-Werner JP, Yakimov BP, Nazarikov G, Drachev VP, Shirshin EA, Ntziachristos V, Stiel AC, Zharov VP, Gorin DA.. Amplification of photoacoustic effect in bimodal polymer particles by self-quenching of indocyanine green. Biomed Opt Express. 2019
10.1364/BOE.10.004775

Mishra K, Fuenzalida-Werner JP, Ntziachristos V, Stiel AC.. Photocontrollable Proteins for Optoacoustic Imaging. Anal Chem. 2019
10.1021/acs.analchem.9b01048

Peters L, Weidenfeld I, Klemm U, Loeschcke A, Weihmann R, Jaeger KE, Drepper T, Ntziachristos V, Stiel AC.. Phototrophic purple bacteria as optoacoustic in vivo reporters of macrophage activity. Nat Commun. 2019
10.1038/s41467-019-09081-5

Gujrati V, Prakash J, Malekzadeh-Najafabadi J, Stiel A, Klemm U, Mettenleiter G, Aichler M, Walch A, Ntziachristos V.. Bioengineered bacterial vesicles as biological nano-heaters for optoacoustic imaging. Nat Commun. 2019
10.1038/s41467-019-09034-y

2018

Fuenzalida-Werner JP, Janowski R, Mishra K, Weidenfeld I, Niessing D, Ntziachristos V, Stiel AC.. Crystal structure of a biliverdin-bound phycobiliprotein: Interdependence of oligomerization and chromophorylation. J Struct Biol. 2018
10.1016/j.jsb.2018.09.013

Vetschera P, Mishra K, Fuenzalida-Werner JP, Chmyrov A, Ntziachristos V, Stiel AC.. Characterization of Reversibly Switchable Fluorescent Proteins in Optoacoustic Imaging. Anal Chem. 2018
10.1021/acs.analchem.8b02599



Before 2017, older publications of AC Stiel

2016

Stiel AC, Nellen M, Höcker B.. PocketOptimizer and the Design of Ligand Binding Sites. Methods Mol Biol. 2016
10.1007/978-1-4939-3569-7_5

2015

Deán-Ben XL, Stiel AC, Jiang Y, Ntziachristos V, Westmeyer GG, Razansky D.. Light fluence normalization in turbid tissues via temporally unmixed multispectral optoacoustic tomography. Opt Lett. 2015
10.1364/OL.40.004691

Stiel AC, Deán-Ben XL, Jiang Y, Ntziachristos V, Razansky D, Westmeyer GG.. High-contrast imaging of reversibly switchable fluorescent proteins via temporally unmixed multispectral optoacoustic tomography. Opt Lett. 2015
10.1364/OL.40.000367

2014

Lavoie-Cardinal F, Jensen NA, Westphal V, Stiel AC, Chmyrov A, Bierwagen J, Testa I, Jakobs S, Hell SW.. Two-color RESOLFT nanoscopy with green and red fluorescent photochromic proteins. Chemphyschem. 2014
10.1002/cphc.201301016

Stiel AC, Feldmeier K, Höcker B.. Identification of protein scaffolds for enzyme design using scaffold selection. Methods Mol Biol. 2014
10.1007/978-1-4939-1486-9_9

2011

Willig KI, Stiel AC, Brakemann T, Jakobs S, Hell SW.. Dual-label STED nanoscopy of living cells using photochromism. Nano Lett. 2011
10.1021/nl202290w

Brakemann T, Stiel AC, Weber G, Andresen M, Testa I, Grotjohann T, Leutenegger M, Plessmann U, Urlaub H, Eggeling C, Wahl MC, Hell SW, Jakobs S.. A reversibly photoswitchable GFP-like protein with fluorescence excitation decoupled from switching. Nat Biotechnol. 2011
10.1038/nbt.1952

2010

Brakemann T, Weber G, Andresen M, Groenhof G, Stiel AC, Trowitzsch S, Eggeling C, Grubmüller H, Hell SW, Wahl MC, Jakobs S.. Molecular basis of the light-driven switching of the photochromic fluorescent protein Padron. J Biol Chem. 2010
10.1074/jbc.M109.086314

2008

Testa I, Schönle A, von Middendorff C, Geisler C, Medda R, Wurm CA, Stiel AC, Jakobs S, Bossi M, Eggeling C, Hell SW, Egner A.. Nanoscale separation of molecular species based on their rotational mobility. Opt Express. 2008
10.1364/oe.16.021093

Stiel AC, Andresen M, Bock H, Hilbert M, Schilde J, Schönle A, Eggeling C, Egner A, Hell SW, Jakobs S.. Generation of monomeric reversibly switchable red fluorescent proteins for far-field fluorescence nanoscopy. Biophys J. 2008
10.1529/biophysj.108.130146

Andresen M, Stiel AC, Fölling J, Wenzel D, Schönle A, Egner A, Eggeling C, Hell SW, Jakobs S.. Photoswitchable fluorescent proteins enable monochromatic multilabel imaging and dual color fluorescence nanoscopy. Nat Biotechnol. 2008
10.1038/nbt.1493

2007

Eggeling, C., Hilbert, M., Bock, H., Ringemann, C., Hofmann, M., Stiel, A. C., Andresen M, Jakobs S, Egner A, Schönle A, Hell, S. W. . Reversible photoswitching enables single‐molecule fluorescence fluctuation spectroscopy at high molecular concentration. Microscopy research and technique. 2007
10.1002/jemt.20505

Egner A, Geisler C, von Middendorff C, Bock H, Wenzel D, Medda R, Andresen M, Stiel AC, Jakobs S, Eggeling C, Schönle A, Hell SW.. Fluorescence nanoscopy in whole cells by asynchronous localization of photoswitching emitters. Biophys J. 2007
10.1529/biophysj.107.112201

Andresen M, Stiel AC, Trowitzsch S, Weber G, Eggeling C, Wahl MC, Hell SW, Jakobs S.. Structural basis for reversible photoswitching in Dronpa. Proc Natl Acad Sci U S A. 2007
10.1073/pnas.0700629104

Stiel AC, Trowitzsch S, Weber G, Andresen M, Eggeling C, Hell SW, Jakobs S, Wahl MC.. 1.8 A bright-state structure of the reversibly switchable fluorescent protein Dronpa guides the generation of fast switching variants. Biochem J. 2007
10.1042/BJ20061401

2005

Andresen M, Wahl MC, Stiel AC, Gräter F, Schäfer LV, Trowitzsch S, Weber G, Eggeling C, Grubmüller H, Hell SW, Jakobs S.. Structure and mechanism of the reversible photoswitch of a fluorescent protein. Proc Natl Acad Sci U S A. 2005
10.1073/pnas.0502772102